DETERMINATION OF BERBERINE AND PALMATINE IN CORTEX PHELLODENDRON AND CHINESE PATENT MEDICINES BY HPLC

Berberine (1)and palmatine (2) in cortex phellodendron and Chinese patent medicines were determined by HPLC. The optimal composition of mobile phase CH₃COOEt—HCOOH—EtOH (15:3: 2) for HPLC separation of berberine and palmatine was successfully determined by using window diagram technique. Detection wavelength was 346nm. Flow rate: 1.5 mlimin. Calibration graphs for 1 and 2 were rectilinear for 0.06～0.32μg and 0.12～0.32μg respectively. The average recoveries of the two corresponding components were found to be over 96% and their coefficients of variation were below 5%.