Influence of combination on the specific chromatogram of Glycyrrhiza in Sini Decoctions by HPLC

The paper is to report the establishment of an HPLC specific chromatogram of Glycyrrhiza in Sini decoctions and the influence of combination on the specific chromatogram.  The RP-HPLC method was used with a Phenomenex Gemini C₁₈ column (250 mm × 4.6 mm ID, 5 μm), and acetonitrile-0.05% trifluoroacetic acid (gradient elution) as mobile phase.  Flow rate was 0.8 mL·min⁻¹ and the detection wavelength was set at 232 nm.  The temperature of column was 30 ℃.  The method is stable and reliable with a good reproducibility, it can be used to determine the specific chromatogram of Glycyrrhiza in Sini Decoctions.  Twenty peaks were selected as specific peaks in Sini Decoction with liquiritin peak as the reference peak.  Six of them were from Glycyrrhiza and the other 6 peaks were from both Glycyrrhiza and Ganjiangfuzi Decoction.  The areas of specific peaks of Sini Decoctions were smaller than those in the chromatogram of Glycyrrhiza.  The specific chromatogram of Glycyrrhiza in Sini Decoctions is markedly influenced by Radix Aconiti Carmichaeli and Rhizoma Zingiberis.  The areas of the specific peaks in Sini Decoctions were reduced obviously.  The method is stable and reliable with a good reproducibility, it can be used to determine the specific chromatogram of Glycyrrhiza in Sini Decoctions.