Identification of metabolites of arbidol by ultra-high performance liquid chromatography tandem mass spectrometry

UPLC-MS-MS system was used for the identification of arbidol metabolites in the rat feces, urine and plasma samples.  The system was so powerful a way with high ability of separation and analysis, based on both chromatography and mass properties.  The isotope of Br was also a good indicator for metabolites finding.  There were altogether 9 metabolites detected and identified, including 2 phase Ⅰ biotransformation products and 7 phase Ⅱ ones.  It is concluded that arbidol mainly undergo metabolic reactions such as N-demethylation, S-oxidation, glucuronidation and sulfation in rats.