Intervention of cetirizine on monocyte chemoattractant protein-1 in cutaneous inflammation

AimTo study the intervention of cetirizine on monocyte chemoattractant protein-1 (MCP-1) in different cutaneous inflammation models.MethodsHistamine and IFN-γ stimulated dermal fibroblast cells and HaCaT cells to mimic cutaneous inflammation.Expression of MCP-1 was assessed by means of RT-PCR and ELISA.ResultsCompared with the control group of dermal fibroblast(DF) cells and HaCaT cells, MCP-1 mRNA was significantly upregulated by histamine (10 μmol·L^-1) and IFN-γ (20 ng·mL^-1).The protein secretions of MCP-1 were increased 3.5 fold and 8.4 fold in DF cells, respectively.The similar tendency was observed in HaCaT cells.The enhancing effects of histamine and IFN-γ on MCP-1 protein production were significantly inhibited by cetirizine (1 and 10 μmol·L^-1) in DF and HaCaT cells.ConclusionCetirizine may exert the anti-inflammatory effect of skin via inhibiting MCP-1 expression.