DETERMINATION OF DIOSGENIN IN DIOSCOREA
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Abstract
A simple and accurate method for the determination of diosgenin in Dioscorea tubers is described.After acid hydrolysis and solvent extraction of the tubers, diosgenin was isolated by thin-layer chromatography. The diosgenin spot was then scraped off and determined by coulometry in a mixture of 1 M KBr-glacial HAc(1: 1).
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