Isolation and identification of Hetisine-type alkaloids from Aconitum coreanum by high speed countercurrent chromatography

AimTo search for more bioactive compounds from the roots of Aconitum coreanum (Lèvl.) Rapaics. MethodsHigh speed countercurrent chromatography was successfully applied to the separation of alkaloids from Aconitum coreanum. The structures were elucidated by their physicochemical properties and spectroscopic analysis. ResultsTwo-phase solvent system composed of CHCl₃-CH₃OH-0.2 mol·L^-1 HCl (10∶3∶3, volume ratio) was used in this experiment, eight alkaloids were obtained from the roots of Aconitum coreanum, which were identified as: 2α-propionyl-11α,13β-diacetyl-14-hydroxyhetisine (I), Guanfu base P (II), Guanfu base G (III), Guanfu base F (IV), Guanfu base Z (V), Guanfu base O (VI), Guanfu base A (VII), Guanfu base B (VIII). ConclusionCompound I is a new alkaloid, named Guanfu base R.