INDUCTION OF NF-κB IN MOUSE PERITONEAL MACROPHAGES BY INFLAMMATORY IRRITANTS

AIM: To establish the model of determining nuclear factor κB（NF-κB） which is stimulated by different inflammatory irritants in inducible peritoneal macrophages from mouse and investigate the inhibitory effect of aspirin on NF-κB activation using this model. METHODS: Lipopolysaccharide(LPS) and phorbol 12-myristate, 13-acetate(PMA) were used as irritants. Electrophoretic mobility shift assay (EMSA) was used as the determination method. RESULTS: LPS(μg.mL^-1， 3 μg.mL^-1) and PMA (1 ng.mL^-1) was shown to increase the content of NF-κB in inducible peritoneal macrophages from the mouse. After the macrophages were treated with aspirin(10^-7 mol.L^-1,　10^-6 mol.L^-1, 10^-5 mol.L^-1),　the increase was inhibited. CONCLUSION: The model of determining NF-κB stimulated by LPS and PMA can be used in researching mechanism of non-steroidal anti-inflammatiory drugs.