STUDIES ON ANALYSIS OF CATECHINSAND ALK ALOI DSINTEABYHPLC

By using HPLC, five catechins (catechin ,epicatechin ,epigallocatechin,epicatechingallate, epigallocatechin gallate )and three tea alkaloids(eaffeine,theobromine, theophylline )wereseparated and determined simultaneously. The chromatographic conditions were as follows: stainlesssteel column, ODS(Nucleosil,10μm),0. 04 mol·L^-1 citricacid solution, dimethyl formamide andtetrahydrofuran (96: 16: 4) as mobile phase,UV detector(at 278 nm). The method is simple,convcnient andsensitive .