THE THERAPEUTIC EFFECT OF O-BIS-(2-CHLOROETHYL)-AMINOMETHYL-PHENYLALANINE DIHYDROCHLORIDE (OCAPHANE,AT-581) ON INTRACEREBRAL GROWTH OF BROWN-PEARCE CARCINOMA IN RABBITS

Brown-Pearce carcinoma was grown successfully with 100% tumor takes when inoculated intracerebrally to rabbits. The average survival times of rabbits inoculated at different sites were as follows: cerebellum-12.4 days, cerberum-11.2 days and thalamus10.8 days. On the 10th day after inoculation the size of tumor mass in thalamus was 21.5 mm², in cerebrum-11.8 mm² and in cerebellum-5.2 mm².Intravenous injections of Ocaphane at the dosage of 0.7 or 1.0 mg/kg/day for 7-10 days significantly prolonged the survival time of rabbits.The percentages of prolongation for cerebellar, cerebral and thalamic tumors were 61～64%,33～38% and 23～30%, respectively. This result suggests that Ocaphane may cross the blood-brain barrier of tumor-bearing rabbits and the different degrees of therapeutic effect may be associated with the different growth rates of the tumor in various regions of the brain.It was demonstrated that when cells of Brown-Pearce carcinoma were exposed to Ocaphane at the concentration of 100 μg/ml for 3 hours, the activity of the reimplanted cells decreased significantly.