INVESTIGATION ON THE METABOLISM OF DEHYDROCHLOROMETHYLTESTOSTERONE IN MAN

A GC-MS method for the investigation on the metabolism of dehydrochloromethyltestosterone (DHCMT)in man is described. Steroids in human urine were adsorbed on a macroporous XAD-2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for derivatization and then analyzed with GC-MS. DHCMT and its 7 metabolites were detected in urine samples 8 to 30 h after oral administration. Their structures and the variation of their concentration in urine were determined and the possible metabolic pathways of DHCMT were proposed on the basis of GC- MS feature of its metabolites. The specific metabolites and characteristic ions for screening DHCMT positive urine were chosen. Furthermore, the influence of the sample pretreatment methods on GC- MS results has been studied.