QUANTITATIVE ANALYSIS OF TRIPCHLOROLIDE IN PHARMACEUTICAL PREPARATION BY RP-HPLC

A reversed phase high performance liquid chromatographic method for the assayof tripchlorolide (T₄) in pharmaceutical preparation was developed. The method used a Nucleosil 5C₁₈ column and a mobile phase of methanol-water (1: 1). The column effluent was monitored at218 nm. T₄ ,triptolide (T₀) and 1,4-dimethoxybenzene (IS) could be separated in less than 25 min.The retention times of T₀, T₄ and IS are 11,17 and 23 min respectively. The method is very simpleand rapid.