PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES OF A GLYCOCONJUGATE SPPA-1 FROM SPIRULINA PLATENSIS

AIM　To isolate polysaccharide from Spirulina platensis and determine its sugar position, molecular weight and biological activities. METHODS　Sephadex G-75 and CM-Sephadex C-50 were used. The sugar position was analyzed by gas chromatography, the molecular weight was determined by GPC. The homogeneity of this glycoconjugate was determined by HPLC and CE. IR and NMR spectra were used to determine the glycosidic linkage. RESULTS　SPPA-1 was a homogeneous glycoconjugate, its carbohydrate chain was composed of α-glucopyranan, carbohydrate content is 91.70%, Nitrogen content was 0.96%, the molecular weight was 69.00×10⁴, SPPA-1 could eliminate O^-₂radicals. CONCLUSION　SPPA-1 was an anti-oxidative glycoconjugate from Spirulina platensis.