Chemical constituents from the twigs and leaves of Harrisonia perforate

This study was performed to investigate the chemical constituents in the twigs and leaves of Harrisonia perforate. Six compounds were isolated from the 95% EtOH extract of the twigs and leaves of Harrisonia perforate by silica gel, ODS, Sephadex LH-20 column chromatographies and preparative HPLC. On the basis of chemical properties and spectra data, these compounds were identified as harriperfin E(1), kihadanin A(2), kihadanin B(3), 6α-acetoxyobacunol acetate(4), gardaubryone C(5), and β-sitosterol methyl ether(6), respectively. Compound 1 is a new chromone, and compounds 2-6 are isolated from this plant for the first time.