Limonoids from fruits of Melia toosendan

 To study the chemical constituents of the fruits of Melia toosendan, three limonoids were isolated and purified by repeated silica gel column chromatography and preparative HPLC from the EtOAc extract of M.toosendan.  Their structures were determined by their physico-chemical properties and spectroscopic data (1D-NMR, 2D-NMR) as: 24, 25, 26, 27-tetranorapotirucalla-(apoeupha)-1α-tigloyloxy-3α, 7α-dihydroxyl-12α- acetoxyl-14, 20, 22-trien-21, 23-epoxy-6, 28-epoxy (1), nimbolinin B (2), and trichilinin D (3), separately.  Compound 1 is a new compound, and compound 2 is obtained from this plant for the first time.