EFFECTS OF CAMPHORAMINE CHLOROACETIC PLATINUM (CCP) ON DNA TEMPLATE

In this paper, the effects of camphoramine chloroacetic platinum (CCP) on DNA template are reported. The mode of action and effects of CCP on DNA secondary structure were analysed by using isotope, UV spectra and viscosity techniques. Experimental results showed that CCP damages DNA template by binding to DNA both covalcntly and noncovalcntly, which further results in DNA interstrand and intrastrand corsslinking, dcstablizing DNA secondary structure such as shortening the molecules, decreasing viscosity, increasing sensitivity to temperature and pH. This suggests that CCP seems to from a cis-bidcntate complex with DNA and finally inhibit DNA replication.