Determinationin vitro of rat plasma protein binding rate of sinomenine by using microdialysis method

AimTo determinein vitro the rat plasma protein binding rate by using microdialysis method. MethodsThe binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (C_f). The plasma protein binding rate was calculated by using the following equation: f=(C₀-C_f)/C₀. ResultThe binding rate was kept relatively stable in the studied concentration range. ConclusionIt is feasible that the plasma protein binding rate can be determined by using microdialysis method.