QUANTITATIVE ANALYSIS OF SOPORIFIC SEDATIVE DRUGS AND LOCAL ANESTHETIC DRUGS WITH UNIDENTICAL STANDARD SUBSTANCE BY GCAED

According to the principle of GC-AED(gas chromatography-atomic emission detector),the possibility of quantitative determination of samples by different substances as internal standard was studied. The element C was determined at wavelength of 496 nm. The relative response factors of 17 soporific sedative and local anesthetic drugs were analyzed with an internal standard of proadifen hydrochloride under optimized analytical conditions. Oven temp.: 45℃(0.5 min)—35℃·min^-1—120℃—15℃·min^-1—220℃—5℃·min^-1—250℃; Cool on column injector temp: 60℃—50℃·min^-1—250℃(0.2 min); inject volume: 1 μl; column head pressure: 145 kPa.The results show that the element C(496) in identical or unidentical substances has similar relative response factor. The RSD is 10.21%, which means that a sample can be quantitatively determined by a different substance when the analysis condition and the selected internal standard are suitable. An amobarbital sample of 109.0 μg·ml^-1 was quantitatively determined by using methylphenobarbitone, pramoxine, lidocaine and itself as internal standard. The results are 117.6, 115.6, 110.0 and 102.14 μg·ml^-1, respectively. They can be quantitatively determined by each other.