Isolation and purification of gonyautoxins from Alexandriun minutum Halim

AimTo isolate and purify gonyautoxins from Alexandriun minutum Halim Amtk2 strain. MethodsThe ethanol extracts of culture Alexandriun minutum Halim Amtk2 were isolated by means of gel filtration chromatography, the toxin fraction obtained was then purified by ion exchange chromatography. Results From 100 liter of cultivation liquid of Alexandriun minutum Halim Amtk2 (6.74±0.31)×10⁹cells were obtained. The ethanol extracts of Alexandriun minutum Halim purified by gel filtration chromatography obtained gonyautoxins mixture 29.59 mg. 4.06 mg of the mixture was further purified by two steps of ion exchange chromatography, and obtained pure GTX-4 (0.40±0.002) mg, GTX-1 (5.95±0.03)×10^-2 mg, GTX-3 (6.92±0.05)×10^-4 mg and GTX-2 (0.11±0.005) mg. ConclusionPure gonyautoxins can be obtained by means of gel filtration chromatography and ion exchange chromatography from ethanol extracts of cultured Alexandriun minutum Halim Amtk2 strain.