ANALYSIS OF PENBUTOLOL AND ITS METABOLITES IN HUMAN BODY FLUID BYGAS CHROMATOGRAPHY-MASS SPECTROMETRY

A method for the detection and identification of penbutolol and its metabolites inhuman body fluid by using gas chromatography-mass spectrometry was established. The urine wasextracted with diethyl ether-isopropanol and the plasma was extracted with Sep-Pas column. AfterTMS derivatization the sample was analyzed with GC-MSD.Six metabolites of Penbutolol wereFound in urine sample. The recovery was 90.83%for urine and 85.88%for plasma. The detectionlimit of penbutolol was 5pg.