DETERMINATION OF EPHEDRINE, PSEUDOEPHEDRINE AND STRYCHNINE IN JIUFEN SAN BY HPLC

An HPLC method was established to separate and determine ephedrine (Ⅰ), pseudoephedrine (Ⅱ) and strychnine (Ⅲ) in Chinese traditional medicine, Jiufen San,on a μ-Bondapak C18 column (10μm, 3.9min×30cm) by using 0.01 mol/L KH₂PO₄-methanol as mobile phase. The programmed gradient elution was carried out and recoveries were determined as 98.94±2.2% for Ⅰ, 97.37±1.9% for Ⅱ and 100.7±1.9% for Ⅲ respectively. An extracting and pretreatment method was designed and the result showed that the extracting efficiency of thins method was 1.33 times (Ⅰ) and 1.29 times (Ⅱ) higherthan those of the Chinese Pharmacopoeia method.