INDUCTION OF CuZn-SOD mRNA EXPRESSION AND ACTIVITY BY PGMS IN RAT LIVER

AIMTo study the effect of propylene glycol mannate sulfate (PGMS) on induction of CuZn-SOD. METHODSWistar rats were given PGMS po at different doses (0, 18.9, 37.8 and 75.6 mg·kg^-1·d) for ten days. Then the rats were sacrificed and the total RNA was extracted from the livers. The total RNA samples were loaded on a 1% agarose gel to detect the quality of total RNA. RT-PCR was applied to study the expression of CuZn-SOD mRNA in rat livers. The amplified products were detected by the 1.5% agarose gel electrophoresis. Simultaneously, the CuZn-SOD activities in rat liver were determined by nitrite method. RESULTSThe total RNA extracted from rat livers was integrated without being decomposed by RNase. The level of CuZn-SOD mRNA of the high-dosage group (75.6 mg·kg^-1·d) was higher than that of the control group (0 mg·kg^-1·d) (P<0.01); the CuZn-SOD activities of the high-dosage group were significantly higher than those of the control group (P<0.001) and the CuZn-SOD activities of the middle-(37.8 mg·kg^-1·d) and low-dosage groups (18.9 mg·kg^-1·d) were higher than those of the control group (P<0.01). CONCLUSIONPGMS can increase the CuZn-SOD activities as well as CuZn-SOD on mRNA level. Therefore, it is possible for PGMS to counteract Atherosclerosis (AS) by inducing the expression of CuZn-SOD.