STUDIES ON CHEMICAL STRUCTURE OF POLYSACCHARIDE FROM FRUIT BODY OF CORIOLUS VERSICOLOR

AIM To study the structure of polysaccharides from Coriolus versicolor. METHODS By chromatography a homogeneous polysaccharide B-1-3 was obtained. The structure of B-1-3 was elucidated using the method of total acid hydrolysis, methylation, and 1D and 2D NMR. RESULTS B-1-3 was found to be mainly composed of Glc by total acid hydrolysis. Its molecular weight was estimated to be 3.16×10⁵ by HPLC method. Methylation analysis and ¹H and ¹³CNMR indicated the main chain of B-1-3 consisted of β-D-1,4-Glc and β-D-1,3-Glc, and branch chains were situated, β-D-1,3,6-Glc and β-D-1,4,6-Glc. ¹H and ¹³CNMR also showed that β-D-Glc-1→3-β-D-Glc-β-D-Glc-1→4-β-D-Glc and β-D-Glc-1→4-β-D-Glc-β-D-Glc-1→4-β-D-Glc fragments existed in B-1-3. CONCLUSION B-1-3 is a glucan which main chain was composed of β-D-1,4-Glc and β-D-1,3-Glc β-D-1,6-Glc.