A novel C27-steroidal glycoside sulfate from Liriope graminifolia

An unusual novel C27-steroidal glycoside sulfate was isolated from the underground organs of Liriope graminifolia (Linn.) Baker with three known compounds.  Their chemical structures were determined by spectral analysis, including HR-MS, 1D and 2D NMR as (25S)-ruscogenin 1-sulfate-3-O-α-L-rhamnopyranoside (1), (25S)-ruscogenin 1-O-β-D-xylopyranosyl-3-O-α-L-rhamnopyranoside (2), hesperidin (3), and 4', 7-dihydroxy- 5-methoxyflavanone (4).  Compound 1 has cytotoxic activities against K562 and HL60 cells with IC₅₀ values of 18.6 μg·mL⁻¹ and 16.5 μg·mL⁻¹, respectively.