Simultaneous determination of 5 kinds of alkaloids in Kechuanning tablets by SPE-UPLC under different UV-vis wavelength

The paper is to establish a method for simultaneous determination of 5 kinds of alkaloids in ephedra and poppy which are in Kechuanning tablets.  Solid-phase extraction (SPE) was adopted in pretreatment, and a UPLC method with 2 different wavelengths had been developed: 210 nm for the detection of morphine, codeine phosphate, ephedrine hydrochloride and pseudoephedrine hydrochloride, and 251 nm for papaverine hydrochloride.  The column used was Acquity UPLC BEH C₁₈ (100 mm × 2.1 mm ID, 1.7 μm) with linear gradient elution using acetonitrile and 0.1% phosphoric acid.  The flow rate was 0.4 mL·min⁻¹, and the column temperature was 30 ℃.  The linear response range was 0.375 0 − 12.50 µg·mL⁻¹ for morphine, 0.064 32 − 2.144 µg·mL⁻¹ for codeine phosphate, 0.030 06 − 1.002 µg·mL⁻¹ for papaverine hydrochloride, 1.126 − 37.52 µg·mL⁻¹ for ephedrine hydrochloride, 0.287 8 − 9.592 µg·mL⁻¹ for pseudoephedrine hydrochloride (r = 0.999 7).  The average recoveries of these compounds were 99.26%, 100.6%, 95.29%, 100.1% and 97.48%, respectively.  This is a more reasonable and credible method of quality control for Kechuanning tablets.