CHEN Qing-wen, SHAN Hong-li, SUN Hong-li, WANG He, YANG Bao-feng. Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytesJ. Acta Pharmaceutica Sinica, 2004, 39(7): 500-503.
Citation: CHEN Qing-wen, SHAN Hong-li, SUN Hong-li, WANG He, YANG Bao-feng. Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytesJ. Acta Pharmaceutica Sinica, 2004, 39(7): 500-503.
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Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytes

    Abstract
  • AimTo determine the effects of cyclovirobuxine D (CD) on intracellular Ca2+ mobilization and L-type Ca2+ current (ICa-L/sub>) in isolated rat cardiomyocytes. MethodsThe effects of CD on the amplitude of ICa-L/sub> and intracellular Ca2+mobilization induced by KCl and caffeine were studied with the method of patch-clamp technique and laser scanning confocal microscopy in rat ventricular myocytes. ResultsCD decreased the amplitude of ICa-L/sub> in a concentration-dependent manner. At 10 mV, 1 and 10 μmol·L-1 CD decreased ICa-L/sub> density from (-9.9±1.8) pA/pF to（-6.4±1.4）and（-4.2±0.6）pA/pF, respectively. Confocal experiments showed that intracellular fluorescent intensity(FI)value of ［Ca2+］i?/sub> in control resting level was not changed by 1 and 10 μmol·L-1 CD. ［Ca2+］i?/sub> increase in response to KCl could not be reduced by CD. The rise of ［Ca2+］i?/sub> in response to caffeine was further enhanced by pretreatment with CD. ConclusionCD decreased ICa,L in a concentration-dependent manner and increased ［Ca2+］i?/sub> release induced by caffeine in rat ventricular cardiomyocytes.
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