Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC

AimTo separate and quantitatively determine six alkaloids: aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi). MethodsA RP-ion-pair HPLC method was established. An AichromBond-1 C₁₈ column was used at a column-temperature of 35 ℃. The mobile phase was CH₃CN-5 mmol·L^-1 NaH₂PO₄ (50∶50) containing 7 mmol·L^-1 SDS at a flow-rate of 1.0 mL·min^-1. The detector was set at UV 235 nm. ResultsThese six alkaloids can be completely separated and determined quantitatively. ConclusionThis method is accurate and suitable for the determination of six alkaloids in Fuzi.