Anticoagulant property of a semi-synthesized sodium β-1,4-glucan sulfate

AimTo investigate the anticoagulant efficacy and mechanism of a semi-synthesized sodium β-1,4-glucan sulfate (Na-MCS). MethodsAnticoagulant activity was evaluated by means of coagulation assays in comparison with heparin. The anticoagulant mechanism of Na-MCS was disclosed by inhibitory analysis of the activities of coagulation factors using chromogenic substrates. Results0.6 μg·mL^-1 Na-MCS could significantly prolong APTT and TT, but has less effect on PT at an even higher concentration. The dosage of Na-MCS required to double APTT of normal human plasma was 0.7 μg·mL^-1, lower than that of heparin with the activity of 150 u·mg^-1. ConclusionNa-MCS represented a potent anticoagulation activity in vitro, which matched the efficacy of heparin in a certain range of concentrations. Na-MCS exhibited anticoagulant activity due to inhibition of the coagulation factors IIa and Xa by the mediation of anti-thrombin AT-III.