DETERMINATION OF PHOSPHOLIPID IN POLYGONUM MULTIFLORUM THUNB. BY SUPERCRITICAL FLUID EXTRACTION—HPLC

AIM： To develop a supercritical fluid extraction (SFE) method for the phospholipids of Polygonum multiflorum Thunb. METHODS: SFE was selected by systemic research method, an HPLC method was developed for the determination of phosphatidylethanolamine and phosphatidylcholine. RESULTS: SFE conditions: pressure 31.5 MPa, temperature 50℃, 0.3 mL.g^-1 modifier. The samples were extracted statically for 5 min, and subsequently extracted dynamically with 7 mL extractant. HPLC conditions: The HPLC column was packed with C₁₈ (5 μm). The mobile phase was methanol—1% phosphoric acid (90∶10). The detection wavelength was 206 nm. CONCLUSION: The method is accurate, convenient and rapid, and can be a measure of quality control for Polygonum multiflorum Thunb. and its preparations.