HPLC fingerprinting of Radix Paeoniae Alba

To establish a sensitive and specific HPLC method for quality control of Radix Paeoniae Alba, HPLC method was applied for quality assessment of Radix Paeoniae Alba. HPLC analysis was performed on a Symmetry C₁₈ column (250 mm×4.6 mm ID, 5 μm, Waters, USA). The mobile phase consisted of acetonitrile (solvent A) and water containing 0.1% (v/v) phosphoric acid (solvent B) at a constant flow rate of 0.8 mL·min^-1. An increasing linear gradient (v/v) of solvent A was used (T/min, %A): (0,10), (5,10), (25,15), (45,22), (46,65), (50,80) and (60,80). The column temperature was set at 25 ℃. The chromatograms were monitored at 230 nm and the on-line UV spectra were recorded in the range of 190-400 nm. The HPLC chromatographic fingerprinting of Radix Paeoniae Alba, showing 11 characteristic peaks, was established from 28 lots of Radix Paeoniae Alba. The areas of main chromatographic peaks were found to complied with the following rule: paeoniflorin>1,2,3,4,6-penta-O-galloyl-glucos>albiflorin>methyl gallate>other compounds. The chromatographic fingerprinting of Radix Paeoniae Alba with high specificity can be used to control its quality and assure lot-to-lot consistency.