LC-MSⁿ analysis of metabolites of 1, 2-bis (1, 2-benzisoselenazolone- 3(2H)-ketone)-ethane, a novel anti-cancer agent in rat

This study is to elucidate the metabolic pathway of 1, 2-bis (1, 2-benzisoselenazolone-3 (2H)- ketone)-ethane (BBSKE) in rats.  Rats were administrated with a single dose of BBSKE 200 mg·kg⁻¹.  The metabolites in rat urine, feces, bile and plasma were identified by LC-MSⁿ analysis.  The characterization  of fragment ions from LC-MSⁿ chromatography and mass spectrometry was applied to the investigation of  structures of metabolites.  Three phase I metabolites were detected in rat urine and feces.  Two of them were also found in plasma and one existed in bile.  These products were derived from oxidized, methylated and S-methylated BBSKE, separately.  One phase II glucuronide of BBSKE was also found in bile.  Therefore, it is possible that BBSKE was metabolized by oxidization, methylation and glucuronidation.