Preparation of solid lipid nanoparticles by solvent diffusion method

Aim To establish an efficient method for preparation of solid lipid nanoparticles with high recovery. Methods Monostearin solid lipid nanoparticles was prepared by solvent diffusion method in aqueous system. The recovery of the method was greatly improved by adjusting the Zeta potential. Results The drug-loaded solid lipid nanoparticles suspension was quickly produced and easily separated with centrifugation at 4 000 r·min^-1 under acidic condition. Compared with the nanoparticles made without adjusting the Zeta potential, the recovery of nanoparticles prepared in this way was significantly increased. The release behavior in vitro showed an initial burst effect in the first 3 hours followed by a slower rate stage of 4 days with nearly 6% drug released in each day. Conclusion The solvent diffusion method in aqueous system might be used as a new method to prepare solid lipid nanoparticles in the future. The loaded drug can be released in a controlled manner.