STUDIES ON THE DETERMINATION OF PLASMA LEVEL AND PHARMACOKINETIC PARAMETERS OF ANISODAMINE BY MICELLAR LIQUID CHROMATOGRAPHY

Anisodamine is a tropane alkaloid isolated from the plant Anisodus,tanguticus, (Max-im) Pasch. It is an anticholine drug widely used in clinics. Micellar liquid chromatography is a newtype of HPLC developed in the 1980's. Direct plasma injection technique is the application of micellarHPLC in bioanalyses. In this paper, a micellar HPLC method, which employs n-propanol as modifier, SDS as surfac-tant, atropine sulphate as internal standard, has been developed. By direct injection, this method wassuccessfully applied to the measurement of plasma level of anisodamine. Application of this method tothe study of anisodamine pharmacokinetics was investigated in human volunteers following a single in-tramuscular injection. The separation was performed in a Shim--pack CLC--CN column (150 mm×6mm ID, 5μm)with a mobile phase of n-propanol--water(15:85) with 45 mmol/L SDS and total ion strength 70mmol/L by adding phosphate, and detected at 205 nm. The standard curve was linear over the concen-tration range of 15～750 ng plasma level (r=0.9972). The measurable lowest concentration was 10ng/ml plasma (S/N=3:1). The study of anisodamine pharmacokinetic in man was also described.