Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase

 Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome.  Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target.  A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP.  Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.