EFFECTS OF PRECURSORS AND FUNGAL ELICITORS ON GKB PRODUCTION IN SUSPENSION CULTURED CELLS OF GINKGO BILOBA L.

AIM: To investigate the effects of precursors and fungal elicitors on the GKB production in suspension cultured cells of Ginkgo biloba L. METHODS: Precursors and fungal elicitors were added to the media. Their effects on the biomass and GKB yields of the suspension of cultured cells were studied. RESULTS: The total GKB yields were enhanced 69%, 13.8% and 11.4% compared with the control by adding 100 mg.L^-1 of isoprene and low concentrations (10 and 50 mg.L^-1) of geraniol in the medium, respectively. Of the 10 investigated fungal elicitors, mycelium extract of Rhizopus japonicus was found to be the best, and at the concentration of 5 mg GE.L^-1, the GKB yield of suspension cells was doubled after the cells were induced for 3 days. CONCLUSION: Adding precursors in the media and applying fungal elicitors were both effective approaches to enhance GKB yields in the suspension culture cell of Ginkgo biloba L.