ISOLATION OF ACTIVE CONSTITUENTS FROM THE FKUITS OF CNIDIUM MONNIERI(L)CUSSON UTILING INCLUSION PHENOMENA BETWEEN HOST AND GUEST MOLECULES

Two active constituents, imperatorin(2 )and xanthoxin(3) , were isolatedsimply and rapidly from the fruits of Cnidium monnieri(L) Cusson utilizing inclusion ability of host molecule 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol.The yields of 2 and 3 were 0.054%and 0.002%respectively.The chemical structures of 2 and 3 were identified by means of UV,IR,¹HNMR and ¹³CNMR and their purities were determined by HPLC,X-ray crystal structural analysisshows that the host molecule forms 1 :2 inclusion compound with 2 by hydrogen bond.The inclusioncrystals beIong to monoclinic system,space group P21/C,a=15.468(5)A,5:b=8. 595(3)A,c=18.663(7)A,β=93.64(5)°,Z=4 and R=0.088.