THE IDENTIFICATION AND DETERMINATION OF MARKER SUBSTANCES IN XIAOYAO WAN BY THREE DIMENSIONAL HPLC
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Abstract
A Chinese traditional patent medicine Xiaoyao Wan was analysed by threedimensinal HPLC. The result shows that the Chinese traditional medicines involved in Xiaoyao Wanwere identified excellently and the content of marker substances can be determined accurately. According to the results of determination, the content of saikosaponin b1, b2, glycyrrhizin,paeoniflorin and atractylenolide Ⅲ in Xiaoyao Wan was 0. 43, 0. 53, 5. 22, 3. 95 and 0. 16(mg/g), respectively); The RSD was 1.2, 0. 8, 0. 4, 0. 3 and 1.0 (%) respectively.
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