DETERMINATION OF COMPOUND PREPARATION CONTAINING UNKNOWN ABSORPTIVE BACKGROUND BY UV SPECTROPHOTOMETRY

A novel algorithm of target factor analysis has been devoloped for detection and correction of unknown absorptive background in multicomponent analysis. The algorithm is based on the property that the estimated spectra can gradually approach the true ones by iterative refinements. Paracetamol and antipyrine contained in compound injection of paracetamol were determined by this method without any preliminary chemical separation. The average recoveries were both 100.0% and the coefficients of variation were 1.1% and 1.0% respectively. The results deafly indicate that the proposed method may also provide a new approach to the analysis of traditional Chinese medicine containing some unknown absorptive components.