DIRECT INJECTION OF PLASMA TO DETERMINE FAMOTIDINE IN PLASMA USING HPLC COLUMN SWITCHING TECHNIQUE

An automated high performance liquid chromatographic switching system is described for the analysis of famotidinc, an H₂-receptor antagonist in plasma. The system used YWG-C18 short precolumn for on-line sample clean-up and a Shimpack CLC-ODS analytical colunm for separation. A solution of 0.2 mol/L acetic acid was used as prctrcatmcnt mobile phase and 14%(v/v) methanol in ammonium acetate (0.2mol/L) was used as analytical mobile phase. The detection was at 267 nm and the detection limit was 12 ng/ml plasma. Plasma sample was diluted with acetic acid (0.2mol/L) andinjected directly on the HPLC system. The procedure of clean-up is simple and automatic. The recovery of clean-up is 84.1%.