RAPID IDENTIFICATION AND QUANTITATION OF BARBITURATES IN PLASMA USING SOLID-PHASE EXTRACTION COMBINED WITH GC-FID AND GC-MS METHOD

A rapid screening method for the quantitation and identification of 7 barbituratesin plasma is presented.The method employs solid- phase extraction at pH 3 using X-5 resin asadsorbent.Identification of each barbiturate is achieved by retention time and GC- MS. Detectionthreshold(0.3～0.5ug·ml^-1 in a 0. 5 ml plasma sample and l ul injection volume),precision(RSD 3.46～6. 41%),and linearity(r>0.99)were satisfactory for blood level monitoring andclinical toxicological applications.