THE SEPARATION AND IDENTIFICATION OF CARDIAC GLYCOSIDES IN THE KERNAL OF CERBERA ODOLLAM

Four cardiac glycosides (Ⅰ, Ⅱ, Ⅲ, Ⅳ) were isolated from the kernal of Cerbera odollam growing in Hai-Nan Island. They were identified as monoacetyl-thevetin B (Ⅰ), thevetin B (Ⅲ), and their 7,8,-epoxide derivatives which were tanghinoside (Ⅱ) and desacetyl-tanghinoslde (Ⅳ).By enzymatic hydrolysis with the enzyme existing in the kernal four monoglycosides, cerberin (Ⅴ), neriifolin (Ⅶ), tanghinin (Ⅵ) and desacetyl-tanghinin (Ⅷ) were obtained respectively.Tanghinoside (Ⅱ), tanghinin (Ⅵ) and desacetyl-tanghinin (Ⅷ) had not been isolated from Gerbera odollam previously.Desacetyl-tanghinoside (Ⅳ) was a new compound.