Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2

The inhibitory ratio (I %) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA).  The dose-effect relationship between concentrations of artificial musk and I % was established.  It was found that artificial musk had obvious inhibitory action on COX-2.  The concentration for 50% of maximum inhibitory effect (IC₅₀) was about 2.26 mg·mL⁻¹.  There was a good relationship between the logarithm concentrations of artificial musk and I % when the concentrations of artificial musk ranged from 0.31−20.0 mg·mL⁻¹.  The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly.  This paper provided a novel method and foundational research for the bioassay of artificial musk.