STUDY ON METABOLITES OF TRIMEBUTINE WITH SOLID PHASE EXTRACTION (SPE) — ¹HNMR COMBINATION

AIM: In order to assess the applicability of combined usage of solid phase extraction (SPE) and nuclear magnetic resonance spectroscopy (NMR) in the study of metabolites, trimebutine was selected, which is a compound composed of two parts by ester linkage. METHODS: With SPE the endogenic compounds were removed efficiently, and ¹HNMR was performed for the eluates. After resolving the structure of each piece, the metabolites were predicted, and the non-metabolized positions were pointed out. RESULTS: The hydrolyzed products were the main metabolites of trimebutine, and the main pathway of the metabolism occurred in the part of butanol. The results agree with those by using isotopic method reported in the literature. CONCLUSION: The experiment showed that if the structures of the pieces were confirmed, and they were fitted together with the matching principle of the peak area integral in one compound, the metabolites could be predicted. This method can provide information of the metabolism and it is convenient in operation.