STUDIES ON LUNG TARGETING GELATIN MICROSPHERES OF CARBOPLATIN

AIM: To improve the treatmemt efficacy and reduce the side effect of carboplatin (CBP), the antitumor drug was incorporated into gelatin microspheres (GMS). METHODS: Lung targeting gelatin microspheres of CBP (CBP-GMS) were prepared by the method of emulsion. The ultraviolet absorption spectrophotometric method was used for the determination of CBP. The method of dynamic dialysis was used in in vitro release of CBP from CBP-GMS. S180 lung neoplasm models were established by iv cancer cell. The number of pulmonary nodules was examined for evaluating the treatment efficacy. RESULTS: The data showed that the mean diameter of CBP-GMS was 13 20 μm, with 91 8% of the microspheres ranging from 5 0 μm to 28 6 μm. The average content of CBP in CBP-GMS was 23 76% and the CBP-GMS were stable for three months stored at 37℃ (relative humidity 75%). The release profile in vitro could be described by first order kinetic equation. The results of therapeutic trials showed that the antitumor effects were significantly increased by injection of CBP-GMS compared with injection of CBP solution in the treatment of mice with lung carcinoma. CONCLUSION: The results indicated that CBP-GMS have a good targeting efficiency in vivo, and the biodegradable CBP-GMS may decrease the side effects of carboplatin and improve its treatment efficacy.