STUDIES ON THE CONSTITUENTS OF SCUTELLARIA SPECIES——Ⅳ. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF GLUCURONYLFLAVONOIDS IN SCUTELLARIA IKONNIKOVII JUZ.

Recently several procedures have been reported for the simultaneous separation of flavonoid glycosides by high performance liquid chromatography (HPLC), and reversed phase column is more suitable than normal phase column. Even so, the simultaneous separation of flavonoids with closely related structures is not easy. We investigated a successful procedure for the simultaneous separation of glucuronyl flavonoids with 5-hydroxyl group by using a reversed phase column combined with gradientelution in the constituent studies of Scutellaria ikonnikovii Juz..We have also studied the relationship between the retention time and the position of hydroxyls and glucuronyls in the chemical structrue.