EFFECT OF DL-3-N-BUTYLPHTHALIDE ON DELAYED NEURONAL DAMAGE AFTER FOCAL CEREBRAL ISCHEMIA AND INTRASYNAPTOSOMES CALCIUM IN RATS

Effect of dl-3-n-butylphthalide on the size of infarction and behavior changes wereinvestiga ted a fter delayed neuronal damage in rats s ubjected to permane nt middle cerebral artervocclusion(MCAo)by the methed of Tamura et al. and the scores of behavior were evaluated by themethed of Bederson et al, The results show that the size of infarct area was significantly reduced 2hafter MCAO following administration of NBP at the dose of 80,160 and 240 mg· kg^-1 ,thepercentage of reduction of infarct area was 49.0%, 69.5%and 85.1%respectively. Neurologicaldeficit was also improved. The size of infarction and the score of neurological deficit were also reducedsignificantly following pretreatment with NBP at the dose of 80 mg·kg^-1 per day for 7 days by theend of the final dosage 24 h before MCAO or at the single dose of l60 mg·kg^-1 1h before MCAO.The results suggest that NBP has therapeutic and preventive effect on stroke and imply that NBP hasthe action of attenuating neuronal damage after delayed cerebral injury. In addition , the level ofcalcium(Ca²⁺i) in rat intrasynaptosomes was determined using fura-2(a fluorescence indicator)technique. It was found that NBP ean not lower the rise ofCa²⁺i induced by 30 mmol·L^-1 KCl,However, the effect of NBP on Ca²⁺i overload induced by excitatory amino acid remains to bestudied.