STUDIES ON ISOLATION, PURIFICATION AND STRUCTURE OF NLC-A OF RHAMNUS HETEROPHYLLUS

A water-soluble gluean, NLC-A, from the roots of Rhamnus heterophyllus Olive was obtained as a homogeneous component as determined by HPLC. NLC-A was shown to compose of D-glucose, and its MW was estimated to be 1.2×10~4.Methylation analysis, peroxidation analysis, Smith degradation and reaction with I₂-KI showed that NLC-A is a (1→4)linked α-D-glucan to which are attached glucosyl side chains at o-6 of the glucosyl residues of the main chain.