Synthesis and anti-tumor activities of N-(aminopyridine) benzamide derivaties

To explore novel histone deacetylase (HDAC) inhibitors with anti-tumor activity, on the basis of preliminary studies, sixteen N-(2-amino-4-pyridine) benzamide derivaties (class A) and sixteen N-(2-amino-3- pyridine) benzamide derivaties (class B) were designed and prepared, and their structures were confirmed by  ¹H NMR and HR-MS individually.  The results showed that 30 target compounds except Ⅴ-20 and Ⅴ-21 had HDACs inhibitory activity and Ⅴ-13, Ⅴ-14, Ⅴ-16 were equal to CI-994 at 200 μmol·L^{−1 in vitro}.  Compounds Ⅴ-30、Ⅴ-31 and Ⅴ-32 exhibited potent inhibitory activities on Hut78、Jurkat E6-1、A549、K562 and MDA-MB-435s.