Molecular identification of raw materials from Lian Qiao Bai Du Wan

Lian Qiao Bai Du Wan was used to study the identification of Chinese patent medicine by molecular marker technique.  DNA was extracted through modified CTAB method.  The psbA-trnH and rbcL sequences were gradient amplified, and PCR products were ligated with the pEASY^TM-T5 vector and then transformed into Trans1-T1 cells, respectively.  Clones were selected randomly and sequenced.  All sequences were analyzed by BlastN and the neighbor-joining (NJ) phylogenetic tree was constructed by MEGA 4.0.  The results showed that nine kinds of medicinal materials can be identified by psbA-trnH sequences, and six kinds of medicinal materials by rbcL sequences from Lian Qiao Bai Du Wan.  Molecular marker technique can stably and accurately distinguish multi-origin medicinal materials in Chinese patent medicine.