Antifungal active triterpene glycosides from sea cucumber Holothuria scabra

To study the new antifungal active triterpene glycosides of sea cucumber Holothuria scabra.  Triterpene glycosides from Holothuria scabra were separated and purified by silica gel chromatography,      reversed-phase silica ge1 chromatography and RP-HPLC.  Their structures were elucidated on the basis of  spectral data and chemical evidence.  Three triterpene glycosides were identified as scabraside A (1), echinoside A (2) and holothurin A₁ (3).  Scabraside A (1) is a new triterpene glycoside, and compounds 2 and 3 were   isolated from Holothuria scabra for the first time.  They showed antifungal activities (1≤MIC₈₀≤16 μg·mL⁻¹).