STUDY ON METABOLITES OF EPERISONE WITH SOLID PHASE EXTRACTION COUPLED WITH ¹HNMR SPECTROSCOPY

AIM　To explore the possibility of identifying the metabolites of eperisone by using solid phase extraction (SPE) and NMR spectrometry. METHODS　The endogenous compounds in rat urine were eliminated with SPE column and ¹HNMR was performed on the eluates for the identification of the metabolites. RESULTS　Three metabolites and one fragment were deduced, two of the metabolites were found for the first time. CONCLUSION　The results showed that, using the above methods, the structures of the metabolites could be elucidated by comparing the changes of specific NMR signals without complete separation of the metabolites. The biotransformation in vivo could also be inferred from the structural fragments found in the NMR spectra.